6CY5
Crystal structure of Signal recognition particle receptor FtsY from Elizabethkingia anophelis in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-09-29 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 |
Unit cell lengths | 49.520, 49.640, 69.710 |
Unit cell angles | 101.18, 99.10, 105.58 |
Refinement procedure
Resolution | 43.430 - 1.700 |
R-factor | 0.158 |
Rwork | 0.157 |
R-free | 0.19360 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.430 | 43.430 | 1.740 |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.048 | 0.037 | 0.243 |
Rmeas | 0.052 | 0.038 | 0.287 |
Total number of observations | 469119 | ||
Number of reflections | 66029 | 752 | 4391 |
<I/σ(I)> | 25.34 | 61.64 | 4.11 |
Completeness [%] | 98.0 | 99.3 | 88.3 |
Redundancy | 7.105 | 10.41 | 3.258 |
CC(1/2) | 0.999 | 0.999 | 0.944 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 290 | tray 291448 H10: MCSG1: 0.1 M HEPES: NaOH, pH 7.5 25 % (w/v) PEG 3350 +4mM GTP/MgCl2: Cryo = 15%EG : ElanA.00983.a.B1 at 20 mg/ml, puck frp4-2 |