6CY4
RNA octamer containing 2'-OMe, 4'- Cbeta-OMe U.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | H 3 |
| Unit cell lengths | 90.438, 90.438, 64.123 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.727 - 1.851 |
| R-factor | 0.2498 |
| Rwork | 0.227 |
| R-free | 0.23120 |
| Structure solution method | SAD |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.13_2992) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.727 | 1.920 |
| High resolution limit [Å] | 1.851 | 1.850 |
| Rmerge | 0.144 | 2.000 |
| Rpim | 0.190 | 0.579 |
| Number of reflections | 16493 | 1637 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 98.4 | |
| Redundancy | 51.3 | |
| CC(1/2) | 0.999 | 0.478 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 1 mM RNA; 40 mM sodium cacodylate, 80 mM sodium chloride, 12 mM potassium chloride, 20 mM magnesium chloride, 12 mM spermine; reservoir 40 % MPD |
