6CXL
anti-HIV-1 Fab 2G12 in complex with glycopeptide 10F5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 137.641, 146.308, 148.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.808 - 3.586 |
R-factor | 0.2201 |
Rwork | 0.219 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.063 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.808 | 3.680 |
High resolution limit [Å] | 3.586 | 3.586 |
Rmerge | 0.296 | 1.000 |
Rmeas | 0.316 | 1.000 |
Rpim | 0.142 | 0.480 |
Number of reflections | 17760 | 1170 |
<I/σ(I)> | 5.8 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 8.5 | 8.6 |
CC(1/2) | 0.920 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.2M lithium sulfate, 17.5% Peg400, 0.1M tris |