6CWT
Hepatitis B core-antigen in complex with Fab e21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-24 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 238.376, 66.523, 104.232 |
| Unit cell angles | 90.00, 110.30, 90.00 |
Refinement procedure
| Resolution | 39.983 - 3.151 |
| R-factor | 0.2346 |
| Rwork | 0.231 |
| R-free | 0.28310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d3c |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.809 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.210 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmeas | 0.180 | 1.450 |
| Number of reflections | 26112 | 2196 |
| <I/σ(I)> | 12.9 | 1.9 |
| Completeness [%] | 97.2 | 82.7 |
| Redundancy | 2.1 | 5.1 |
| CC(1/2) | 0.940 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% (w/v) PEG 3350, 0.2 M Potassium thiocyanate 0.1 M Bis-tris propane, pH 6.5 |
