6CVR

Human Aprataxin (Aptx) S242N bound to RNA-DNA, AMP and Zn product complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	40.616, 116.056, 117.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.390 - 1.880
R-factor	0.1673
Rwork	0.166
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ndf
RMSD bond length	0.009
RMSD bond angle	1.001
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.880	1.880
Number of reflections	44982
<I/σ(I)>	17
Completeness [%]	97.3
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	200 mM ammonium formate, 20% w/v polyethylene glycol 3350