6CTZ
Structure of the GDP and kanamycin complex of APH(2")-IIia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.169, 58.982, 70.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.090 - 1.340 |
R-factor | 0.147 |
Rwork | 0.146 |
R-free | 0.17100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tdw |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.086 | 1.360 |
High resolution limit [Å] | 1.340 | 1.340 |
Number of reflections | 73255 | |
<I/σ(I)> | 22.1 | 2.7 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 14.3 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.25 M magnesium chloride, 0.1 M Tris-HCl, 20% PEG4000 |