6CQD
Crystal structure of HPK1 in complex with ATP analogue (AMPPNP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2017-09-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.886, 51.882, 107.500 |
Unit cell angles | 90.00, 131.03, 90.00 |
Refinement procedure
Resolution | 46.987 - 2.120 |
R-factor | 0.2059 |
Rwork | 0.203 |
R-free | 0.24980 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.847 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.127 |
High resolution limit [Å] | 2.120 | 2.120 |
Number of reflections | 35007 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.9 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 0.1 M HEPES, pH 7.5, 12% PEG8000 |