6CQ1
BCL6 BTB domain in complex with 15a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | .987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.012, 39.621, 55.225 |
| Unit cell angles | 83.65, 73.63, 66.98 |
Refinement procedure
| Resolution | 36.466 - 1.699 |
| R-factor | 0.182696736663 |
| Rwork | 0.181 |
| R-free | 0.21573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r29 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.432 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | phenix.refine (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.470 | 1.760 |
| High resolution limit [Å] | 1.690 | 1.700 |
| Number of reflections | 22269 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 86.6 | |
| Redundancy | 2 | |
| CC(1/2) | 0.993 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | .2 M NaCl, .1 M sodium cacodylate pH 6.0, 8% (w/v) PEG-8,000 |
