6CPL
Crystal structure of DR11 presenting the gag293 epitope
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.984 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 65.746, 65.746, 222.514 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.160 - 2.450 |
R-factor | 0.225 |
Rwork | 0.224 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mdo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.160 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rpim | 0.052 | 0.435 |
Number of reflections | 20598 | 2328 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 100.0 | |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG 3350, Tris pH 8.5, Ethylene glycol |