Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8337 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.530, 58.530, 151.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.200 |
| R-factor | 0.1037 |
| Rwork | 0.103 |
| R-free | 0.11710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1thw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.036 | 0.522 |
| Number of reflections | 82240 | 5579 |
| <I/σ(I)> | 26.5 | 2.05 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 7.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.5 | 293 | Na-tartrate (0.52M), Na-ADA (0.1M), agarose gel (0.15% m/v). THE CRYSTALS WERE GROWN IN THE ADVANCED PROTEIN CRYSTALLIZATION FACILITY (APCF) ABOARD THE US SPACE SHUTTLE (MISSION STS-95) USING EITHER A DIALYSIS SETUP (3 CRYSTALS) OR A FREE-INTERFACE DIFFUSION SETUP (1 CRYSTAL)., pH 6.5, Dialysis in APCF reactor, temperature 293K |
