6CNH

Human PRPF4B in complex with Rebastinib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.968610
Spacegroup name	C 1 2 1
Unit cell lengths	85.169, 75.661, 68.075
Unit cell angles	90.00, 92.82, 90.00

Refinement procedure

Resolution	67.990 - 2.000
R-factor	0.18341
Rwork	0.182
R-free	0.20591
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ijp
RMSD bond length	0.008
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.990	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.070	0.535
Rmeas	0.084	0.638
Rpim	0.045	0.344
Number of reflections	29242	2136
<I/σ(I)>	9.7	2.4
Completeness [%]	99.5	99.5
Redundancy	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	18% PEG Smear High, 0.1 M N-(2-acetamido)iminodiacetic acid, 0.2 M ammonium sulfate