6CNH
Human PRPF4B in complex with Rebastinib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.968610 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.169, 75.661, 68.075 |
| Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
| Resolution | 67.990 - 2.000 |
| R-factor | 0.18341 |
| Rwork | 0.182 |
| R-free | 0.20591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ijp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.990 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.535 |
| Rmeas | 0.084 | 0.638 |
| Rpim | 0.045 | 0.344 |
| Number of reflections | 29242 | 2136 |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 18% PEG Smear High, 0.1 M N-(2-acetamido)iminodiacetic acid, 0.2 M ammonium sulfate |
