6CKO
Crystal structure of an AF10 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.701, 122.558, 65.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.700 - 2.000 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.23310 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.081 | 1.372 |
| Rmeas | 0.088 | 1.500 |
| Rpim | 0.032 | 0.592 |
| Total number of observations | 206554 | |
| Number of reflections | 28326 | 1780 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.3 | 89.8 |
| Redundancy | 7.3 | 5.9 |
| CC(1/2) | 0.999 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 1.5 M ammonium sulfate, 0.1 M Tris |
