6CJP

Candida albicans Hsp90 nucleotide binding domain in complex with radicicol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	120
Detector technology	CCD
Collection date	2015-10-10
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	40.530, 80.110, 78.200
Unit cell angles	90.00, 105.09, 90.00

Refinement procedure

Resolution	39.200 - 2.600
Rwork	0.203
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cji
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.054	0.170
Number of reflections	14455	1855
<I/σ(I)>	17	5.9
Completeness [%]	96.9	86
Redundancy	4.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298.15	100mM Hepes 5mM CoCl2 12% PEG 3350