6CJE
Crystal Structure of Mnk2-D228G in complex with Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.915, 105.915, 72.658 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.780 - 3.360 |
R-factor | 0.21629 |
Rwork | 0.212 |
R-free | 0.31573 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hw7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.883 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.440 |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 6977 | |
<I/σ(I)> | 4 | |
Completeness [%] | 98.0 | |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300.15 | 20-30% (w/v) polyacrylic acid 5100, 1-5% PEG 400, and 50 mM Hepes (pH 7.5) |