6CJ5
Crystal Structure of Mnk2-D228G in Complex With Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.370, 105.370, 72.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.700 - 2.800 |
R-factor | 0.2073 |
Rwork | 0.204 |
R-free | 0.28002 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.891 |
Data reduction software | iMOSFLM |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 11166 | |
<I/σ(I)> | 14 | |
Completeness [%] | 99.0 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300.15 | 20-30% (w/v) polyacrylic acid 5100, 1-5% PEG 400, and 50 mM Hepes (pH 7.5) |