6CIT

Crystal Structure of MVM NS2 NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.570, 106.570, 304.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.226 - 2.027
R-factor	0.1885
Rwork	0.188
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb2
RMSD bond length	0.002
RMSD bond angle	0.504
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.027	2.027
Rmerge	0.071	1.327
Rpim	0.023	0.460
Number of reflections	114290	5641
<I/σ(I)>	31.4
Completeness [%]	100.0	100
Redundancy	10.1	9
CC(1/2)		0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	17% PEG3350, 100 MM Bis-Tris, pH 6.4, 200 mM ammonium nitrate