6CIL

PRE-REACTION COMPLEX, RAG1(E962Q)/2-INTACT/INTACT 12/23RSS COMPLEX IN MN2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-11
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	157.332, 118.830, 198.960
Unit cell angles	90.00, 100.59, 90.00

Refinement procedure

Resolution	37.980 - 4.150
R-factor	0.2073
Rwork	0.206
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cik
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.220
High resolution limit [Å]	4.150	4.150
Rmerge	0.091	0.933
Rmeas	0.104	1.067
Rpim	0.051	0.511
Number of reflections	27342	1345
<I/σ(I)>	13.6	1.5
Completeness [%]	99.7	99.7
Redundancy	4.1	4.1
CC(1/2)		0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	277	18% PEG3350, 200 mM KNO3, 50 mM HEPES pH 6.9