6CI7
The structure of YcaO from Methanopyrus kandleri bound with AMPPCP and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-08-13 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.193, 106.755, 141.446 |
| Unit cell angles | 90.00, 103.66, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.18705 |
| Rwork | 0.185 |
| R-free | 0.22643 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.442 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.500 | 2.359 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Number of reflections | 155349 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 98.1 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282.1 | 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M sodium citrate (tribasic), 0.02 M sodium potassium tartrate, 0.02 M sodium oxamate, 0.05 M HEPES pH=7.5, 0.05 M MOPS pH=7.5, 20% glycerol, and 10% PEG 4000 |
