6CHR

Crystal structure of a group II intron lariat with an intact 3' splice site (pre-2s state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	165.015, 256.893, 137.232
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.077 - 3.700
R-factor	0.2191
Rwork	0.217
R-free	0.25290
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.000	3.820
High resolution limit [Å]	3.700	3.700
Number of reflections	31200	2986
<I/σ(I)>	15.1	1.21
Completeness [%]	97.1	92.9
Redundancy	3.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	303	0.4 mM spermine, 21% 2-Methyl-2,4-pentanediol, 175 mM magnesium acetate tetrahydrate, and 90 mM MES monohydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 303K