6CH7
XFEL crystal structure of a natively-glycosylated BG505 SOSIP.664 HIV-1 Envelope Trimer in complex with the broadly-neutralizing antibodies BG18 and 35O22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE MFX |
Synchrotron site | SLAC LCLS |
Beamline | MFX |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.301 |
Spacegroup name | H 3 2 |
Unit cell lengths | 238.944, 238.944, 354.014 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.030 - 3.800 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.26200 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.160 |
Data reduction software | cctbx.xfel |
Data scaling software | cctbx.prime |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.570 | 3.870 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.535 | |
Number of reflections | 38101 | 1759 |
<I/σ(I)> | 4.31 | |
Completeness [%] | 99.1 | 93.2 |
Redundancy | 9.31 | |
CC(1/2) | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5% Tacismate pH 8.0, 14% PEG 3350, 0.1M Tris pH 8.0 |