6CGO
Molecular basis for condensation domain-mediated chain release from the enacyloxin polyketide synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 103.630, 58.770, 107.000 |
Unit cell angles | 90.00, 104.96, 90.00 |
Refinement procedure
Resolution | 51.687 - 2.000 |
R-factor | 0.1811 |
Rwork | 0.180 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.176 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.100 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.107 | 0.332 |
Rmeas | 0.126 | 0.388 |
Rpim | 0.066 | 0.199 |
Number of reflections | 84342 | 12286 |
<I/σ(I)> | 6.8 | 2.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.6 | 3.7 |
CC(1/2) | 0.986 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.17-0.21 diammonium phosphate and 22-25% PEG 3,350 |