6CFV

Structure of Human alpha-Phosphomannomutase 1 in complex with Inosine Monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97917
Spacegroup name	P 43 21 2
Unit cell lengths	52.169, 52.169, 214.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.880 - 1.918
R-factor	0.1766
Rwork	0.172
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fuc
RMSD bond length	0.007
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.880	1.966
High resolution limit [Å]	1.918	1.918
Number of reflections	23377
<I/σ(I)>	30.32
Completeness [%]	98.2
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	20% polyethylene glycol 3350, 0.15 M DL-malate, pH 7.0, 50 mM MgCl2, and 8 mM beta-mercaptoethanol