6CFK

Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	210.090, 449.120, 621.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	121.919 - 2.700
R-factor	0.2082
Rwork	0.206
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.004
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	121.919	121.919	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.146	0.019	1.398
Rmeas	0.173	0.023	1.662
Total number of observations	5338935
Number of reflections	1575457	17861	115660
<I/σ(I)>	8.7	45.27	0.91
Completeness [%]	99.2	95.1	98.8
Redundancy	3.389	3.268	3.339
CC(1/2)	0.992	0.999	0.194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME