6CFH

SWGMMGMLASQ segment from the low complexity domain of TDP-43

Experimental procedure

Source type	TRANSMISSION ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-08-18
Detector	TVIPS F416 CMOS CAMERA
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	8.560, 9.600, 39.970
Unit cell angles	97.17, 92.89, 105.94

Refinement procedure

Resolution	13.170 - 1.500
R-factor	0.283
Rwork	0.280
R-free	0.31300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	13.170	13.170	1.550
High resolution limit [Å]	1.500	6.380	1.500
Rmerge	0.208	0.132	0.855
Rmeas	0.231	0.147	1.096
Total number of observations	7695
Number of reflections	1819	25	168
<I/σ(I)>	3.31	7.43	0.68
Completeness [%]	93.5	86.2	89.4
Redundancy	4.23	5.32	2.369
CC(1/2)	0.987	0.997	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	7.5	303	phosphate buffered saline, shaken for 80 hours