6CFD
ADEP4 bound to E. faecium ClpP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.395, 202.166, 97.385 |
| Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
| Resolution | 101.080 - 2.570 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sta |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.795 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.163 | 0.086 | 0.878 |
| Rmeas | 0.178 | 0.094 | 0.970 |
| Rpim | 0.069 | 0.036 | 0.405 |
| Total number of observations | 688990 | ||
| Number of reflections | 114674 | 5813 | 5793 |
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 6 | 6.2 | 5 |
| CC(1/2) | 0.994 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291.15 | 0.1 M sodium acetate, pH 4.5, 18-35% MPD, 0.02 M calcium chloride |
