6CF4
Segment NFGTFS, with familial mutation A315T and phosphorylated threonine, from the low complexity domain of TDP-43, residues 312-317
Experimental procedure
Source type | TRANSMISSION ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-01-09 |
Detector | TVIPS F416 CMOS CAMERA |
Wavelength(s) | 0.0251 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 23.650, 4.720, 30.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.645 - 0.750 |
R-factor | 0.2346 |
Rwork | 0.232 |
R-free | 0.25120 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.042 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 7.645 | 7.645 | 0.770 |
High resolution limit [Å] | 0.750 | 3.360 | 0.750 |
Rmerge | 0.172 | 0.068 | 0.661 |
Rmeas | 0.203 | 0.082 | 0.757 |
Total number of observations | 15891 | ||
Number of reflections | 4177 | 55 | 314 |
<I/σ(I)> | 3.9 | 9.55 | 1.28 |
Completeness [%] | 86.6 | 70.5 | 87.2 |
Redundancy | 3.804 | 2.909 | 4.01 |
CC(1/2) | 0.989 | 0.991 | 0.599 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch | 7.5 | 310 | 1X PBS 7.5 |