6CF4

Segment NFGTFS, with familial mutation A315T and phosphorylated threonine, from the low complexity domain of TDP-43, residues 312-317

Experimental procedure

Source type	TRANSMISSION ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-01-09
Detector	TVIPS F416 CMOS CAMERA
Wavelength(s)	0.0251
Spacegroup name	P 21 21 21
Unit cell lengths	23.650, 4.720, 30.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.645 - 0.750
R-factor	0.2346
Rwork	0.232
R-free	0.25120
RMSD bond length	0.021
RMSD bond angle	2.042
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.645	7.645	0.770
High resolution limit [Å]	0.750	3.360	0.750
Rmerge	0.172	0.068	0.661
Rmeas	0.203	0.082	0.757
Total number of observations	15891
Number of reflections	4177	55	314
<I/σ(I)>	3.9	9.55	1.28
Completeness [%]	86.6	70.5	87.2
Redundancy	3.804	2.909	4.01
CC(1/2)	0.989	0.991	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	7.5	310	1X PBS 7.5