6CCB
Crystal structure of 253-11 SOSIP trimer in complex with 10-1074 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03327 |
Spacegroup name | H 3 |
Unit cell lengths | 237.284, 237.284, 282.044 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.547 - 6.500 |
R-factor | 0.3047 |
Rwork | 0.298 |
R-free | 0.36580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t3x |
RMSD bond length | 0.002 |
RMSD bond angle | 0.539 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.600 | 6.740 |
High resolution limit [Å] | 6.500 | 6.500 |
Rmerge | 0.070 | 0.560 |
Rpim | 0.050 | 0.400 |
Number of reflections | 11600 | 5468 |
<I/σ(I)> | 11.9 | |
Completeness [%] | 99.2 | |
Redundancy | 2.9 | |
CC(1/2) | 0.996 | 0.491 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5 M sodium chloride, 1.9 M ammonium sulphate and 0.1 M sodium cacodylate, pH 6.5 |