6CC8
Crystal structure MBD3 MBD domain in complex with methylated CpG DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.550, 36.640, 130.930 |
Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
Resolution | 35.730 - 1.950 |
R-factor | 0.226 |
Rwork | 0.224 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6C2K |
RMSD bond length | 0.014 |
RMSD bond angle | 1.652 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.730 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.074 | 0.761 |
Number of reflections | 25160 | |
<I/σ(I)> | 14 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 5.3 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M HEPES, 5% ethylene glycol |