6CC8
Crystal structure MBD3 MBD domain in complex with methylated CpG DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.550, 36.640, 130.930 |
| Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
| Resolution | 35.730 - 1.950 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6C2K |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.652 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.730 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.074 | 0.761 |
| Number of reflections | 25160 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M HEPES, 5% ethylene glycol |
