6CBP

Crystal structure of the single chain variable fragment of the DH270.6 bnAb in complex with the Man9-V3 glycopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97925
Spacegroup name	P 41 2 2
Unit cell lengths	68.005, 68.005, 128.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.141 - 2.170
R-factor	0.224
Rwork	0.221
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tqa
RMSD bond length	0.006
RMSD bond angle	0.934
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.141	2.248
High resolution limit [Å]	2.170	2.170
Number of reflections	15878	5929
<I/σ(I)>	7.65
Completeness [%]	95.1
Redundancy	4.9
CC(1/2)	0.982	0.417

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	30% PEG8000, 10 mM CHES, 1 M sodium chloride, pH 9.0