6CB1

Yeast nucleolar pre-60S ribosomal subunit (state 3)

EM specimen

VitrificationYES
StainingNO
InstrumentFEI VITROBOT MARK IV

EM Data Collection

MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
DetectorGATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)4.6
Software,SerialEM,CTFFIND,Coot,RELION,PHENIX
SymmetryC1
CTF correctionNONE