6CAP

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus in complex with Sisomicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	400.970, 400.970, 175.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.908 - 3.400
R-factor	0.1833
Rwork	0.183
R-free	0.22980
RMSD bond length	0.004
RMSD bond angle	0.823
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.908	39.908	3.200
High resolution limit [Å]	3.120	13.950	3.120
Rmerge	1.011	0.065
Rmeas	1.017	0.066
Total number of observations	23977077
Number of reflections	244903	2916	13000
<I/σ(I)>	7.64	61.58	0.19
Completeness [%]	97.7	93.6	70.9
Redundancy	97.904	90.498	43.218
CC(1/2)	0.997	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD