6C8W

Crystal structure of Aspartate Semialdehyde Dehydrogenase with NADP from Blastomyces dermatitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	107.460, 107.460, 223.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.836 - 2.600
R-factor	0.1969
Rwork	0.195
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hsk
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.060	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	46721	46721
<I/σ(I)>	9.1	2
Completeness [%]	100.0	100
Redundancy	9.7	9.7
CC(1/2)	0.996	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2M Ammonium Citrate Tribasic pH 7.0, 18%w/v PEG3350