6C7O

Crystal structure of D477G ACO/RPE65 chimera, trigonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 3 1 2
Unit cell lengths	126.520, 126.520, 159.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.580 - 3.150
R-factor	0.23327
Rwork	0.232
R-free	0.26579
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c7k
RMSD bond length	0.006
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.230
High resolution limit [Å]	3.150	3.150
Rmerge	0.125	2.916
Number of reflections	25537	4090
<I/σ(I)>	16
Completeness [%]	99.9	100
Redundancy	18.6	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	281	0.1 M Bis-Tris propane, pH 6 and 21% w/v sodium polyacrylate 2100
1	VAPOR DIFFUSION, HANGING DROP	6	281	0.1 M Bis-Tris propane, pH 6 and 21% w/v sodium polyacrylate 2100