6C7A
Conformational Changes in a Class A Beta lactamase that Prime it for Catalysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2015-01-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.825, 71.825, 96.534 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.270 - 1.050 |
| Rwork | 0.120 |
| R-free | 0.15000 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.075 | 0.370 |
| Rpim | 0.020 | 0.149 |
| Number of reflections | 134116 | |
| <I/σ(I)> | 5.8 | 2 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 9.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.1 | 293 | 10 mg/ml protein in a solution containing 2.0 M ammonium sulfate and 0.1 M sodium citrate (pH 6.1) |
