6C6W
Structure of a Complex Between Thaumatin and Thioflavin T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.475, 58.475, 151.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.560 - 2.230 |
| R-factor | 0.13166 |
| Rwork | 0.130 |
| R-free | 0.16690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.560 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.187 |
| Rmeas | 0.071 | 0.247 |
| Rpim | 0.021 | 0.159 |
| Number of reflections | 12192 | 247 |
| <I/σ(I)> | 23.1 | 2.9 |
| Completeness [%] | 88.1 | 20.4 |
| Redundancy | 9.5 | 1.3 |
| CC(1/2) | 0.999 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Sitting drop composed of equal parts 40 mg/ml protein in H20 and 1.0 M sodium-potassium tartrate at pH 6.5. Crystals grew in 24 to 48 hours at room temperature |
