6C63
Crystal Structure of the Mango-II Fluorescent Aptamer Bound to TO1-Biotin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.495 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 36.831, 182.412, 107.494 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.206 - 2.900 |
R-factor | 0.189598873662 |
Rwork | 0.185 |
R-free | 0.22955 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v3f |
RMSD bond length | 0.007 |
RMSD bond angle | 1.370 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.206 | 2.990 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.058 | 0.340 |
Number of reflections | 15464 | 1257 |
<I/σ(I)> | 4.4 | |
Completeness [%] | 99.0 | 99 |
Redundancy | 8.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES pH 7.5, 2.45 M Ammonium Formate, 14.5% glycerol, 6.5% D-Sorbitol |