6C5A
Human UDP-Glucose Dehydrogenase with UDP- Glc and NADH bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2017-06-20 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 95.450, 199.870, 200.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.751 - 2.300 |
| R-factor | 0.1939 |
| Rwork | 0.193 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.034 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.854 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.116 | 1.844 |
| Number of reflections | 170376 | 12480 |
| <I/σ(I)> | 12.18 | 1.33 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.43 | 7.59 |
| CC(1/2) | 0.998 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 13% PEG3350; 50 mM dipotassium phosphate, 100 mM Tris |
