6C5A
Human UDP-Glucose Dehydrogenase with UDP- Glc and NADH bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2017-06-20 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 95.450, 199.870, 200.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.751 - 2.300 |
R-factor | 0.1939 |
Rwork | 0.193 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.034 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.854 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.116 | 1.844 |
Number of reflections | 170376 | 12480 |
<I/σ(I)> | 12.18 | 1.33 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.43 | 7.59 |
CC(1/2) | 0.998 | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 13% PEG3350; 50 mM dipotassium phosphate, 100 mM Tris |