6C58
Human UDP-Glucose Dehydrogenase A225L substitutuion with UDP-xylose bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2015-11-14 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.520, 194.990, 109.540 |
Unit cell angles | 90.00, 110.71, 90.00 |
Refinement procedure
Resolution | 51.288 - 2.198 |
R-factor | 0.2124 |
Rwork | 0.209 |
R-free | 0.24440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ptz |
RMSD bond length | 0.005 |
RMSD bond angle | 0.557 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.288 | 2.250 |
High resolution limit [Å] | 2.198 | 2.198 |
Rmeas | 0.154 | 1.119 |
Number of reflections | 178670 | 13090 |
<I/σ(I)> | 5.95 | |
Completeness [%] | 96.3 | 95.8 |
Redundancy | 2.82 | 2.76 |
CC(1/2) | 0.992 | 0.436 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 10% PEG3350, 16% 1,5-pentanediol, 100 mM HEPES |