6C4X
Cross-alpha Amyloid-like Structure alphaAmmem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.11584 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.676, 82.676, 155.009 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.600 - 3.550 |
| R-factor | 0.30948 |
| Rwork | 0.307 |
| R-free | 0.34514 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.368 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | 3.770 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Number of reflections | 7070 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 97.9 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 35% MPD, 0.2 M MgCl2 and imidazole 0.1 M pH 8 |
