6C3I
Crystal structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter G45R mutant in an inward occluded state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97930 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.727, 97.850, 118.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.619 - 2.950 |
R-factor | 0.244270636381 |
Rwork | 0.243 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kte |
RMSD bond length | 0.002 |
RMSD bond angle | 0.478 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 2.950 | 3.000 |
Rmerge | 0.233 | 1.388 |
Rmeas | 0.270 | 1.608 |
Rpim | 0.133 | 0.795 |
Number of reflections | 17206 | 842 |
<I/σ(I)> | 6.4 | 0.95 |
Completeness [%] | 98.5 | 98.8 |
Redundancy | 3.9 | 4 |
CC(1/2) | 0.386 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 298 | 20% PEG550 MME, 150 mM sodium chloride, 100 mM HEPES, pH 7.0 |