6C1P

HypoPP mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.99994
Spacegroup name	P 21 2 21
Unit cell lengths	125.500, 125.552, 192.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.458 - 2.900
R-factor	0.2388
Rwork	0.235
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.012
RMSD bond angle	1.703
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	67766
<I/σ(I)>	14.5
Completeness [%]	98.0
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	ammonium sulphate, Na-citrate