6C1O
FGFR1 kinase domain complexed with FIIN-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95365 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.030, 50.384, 65.513 |
| Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
| Resolution | 101.300 - 2.290 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ur1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.300 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rpim | 0.031 | 0.844 |
| Number of reflections | 29542 | 2790 |
| <I/σ(I)> | 13.3 | 0.8 |
| Completeness [%] | 97.9 | 89.3 |
| Redundancy | 15.5 | 9.1 |
| CC(1/2) | 0.998 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% MPEG 5000, 0.1 M sodium cacodylate pH 7.5, 0.2 M ammonium sulfate |
