6C0U
Crystal structure of cAMP-dependent protein kinase Calpha subunit bound with N46
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2017-05-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.494, 120.494, 80.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.833 - 2.650 |
| R-factor | 0.2113 |
| Rwork | 0.208 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bx6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.544 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.086 | |
| Number of reflections | 20021 | |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.8 | |
| CC(1/2) | 0.999 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 16% (w/v) PEG8000, 0.04 M potassium phosphate (monobasic), 20% (v/v) glycerol |
