6BZ1
MEF2 Chimera D83V mutant/DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-02-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 32 |
Unit cell lengths | 78.440, 78.440, 111.041 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.990 - 2.970 |
R-factor | 0.14091 |
Rwork | 0.139 |
R-free | 0.18997 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.525 |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.930 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 15258 | |
<I/σ(I)> | 41.66 | |
Completeness [%] | 99.7 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1M MES pH 5.94, 0.2 M NaCl and 18% PEG2000MME |