Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

6BZ0

1.83 Angstrom Resolution Crystal Structure of Dihydrolipoyl Dehydrogenase from Acinetobacter baumannii in Complex with FAD.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]100
Detector technologyCCD
Collection date2017-10-17
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.97872
Spacegroup nameP 1
Unit cell lengths57.965, 82.769, 96.262
Unit cell angles80.38, 80.21, 89.49
Refinement procedure
Resolution29.630 - 1.830
R-factor0.18125
Rwork0.179
R-free0.22202
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5u8u
RMSD bond length0.008
RMSD bond angle1.395
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwareMoRDa
Refinement softwareREFMAC (5.8.0189)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0001.860
High resolution limit [Å]1.8301.830
Rmerge0.0800.803
Rmeas0.0930.928
Rpim0.0470.466
Number of reflections1504037400
<I/σ(I)>15.22.1
Completeness [%]97.696.2
Redundancy3.94
CC(1/2)0.696
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.5293Protein: 22.6 mg/ml, 0.5M Sodium chloride, 0.01M Tris HCl (pH 8.3); Screen: Classics II (G10), 0.2M Magnesium chloride, 0.1M Bis-Tris (pH 5.5), 25% (w/v) PEG 3350.

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon