6BY8
Menin in complex with MI-1482
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0331 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.991, 80.222, 124.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.520 - 1.900 |
R-factor | 0.1524 |
Rwork | 0.150 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x5y |
RMSD bond length | 0.008 |
RMSD bond angle | 0.882 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.082 | 0.508 |
Number of reflections | 54917 | |
<I/σ(I)> | 12.48 | |
Completeness [%] | 100.0 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.2 M ammonium acetate, 0.1 M HEPES and 25% w/v PEG 3,350. This solution was mixed 1:1 with 2.5mg/mL protein in 50mM Tris-HCl, 50mM NaCl, and 1mM TCEP. Prior to data collection, crystals were transferred into a cryo-solution containing 20% PEG550 MME and flash-frozen in liquid nitrogen |