6BWV
Crystal Structure of the 4-1BB/4-1BBL Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-05 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 0.97946 |
Spacegroup name | I 21 3 |
Unit cell lengths | 161.571, 161.571, 161.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.000 - 2.400 |
R-factor | 0.22799 |
Rwork | 0.227 |
R-free | 0.26479 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x29 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.122 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.165 | 1.449 |
Rmeas | 0.173 | 1.523 |
Rpim | 0.053 | 0.467 |
Number of reflections | 27518 | 2863 |
<I/σ(I)> | 12.4 | 2.5 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 20.6 | 10.3 |
CC(1/2) | 0.994 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Potassium citrate tribasic monohydrate 20 % w/v PEG 3350 |