6BW3

Crystal structure of RBBP4 in complex with PRDM3 N-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-10
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	75.979, 59.822, 101.773
Unit cell angles	90.00, 94.55, 90.00

Refinement procedure

Resolution	46.950 - 2.200
R-factor	0.21671
Rwork	0.215
R-free	0.24594
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xu7
RMSD bond length	0.011
RMSD bond angle	1.476
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.950	46.950	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.099	0.021	0.991
Rmeas	0.114	0.025	1.141
Rpim	0.056	0.014	0.559
Number of reflections	46303	702	3952
<I/σ(I)>	14.3
Completeness [%]	99.4	97.9	98.4
Redundancy	4.1	3.7	4
CC(1/2)	0.990	0.931	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	20% PEG3350, 0.2 M sodium malonate, 0.1 M BisTris