6BV4
Crystal structure of porcine aminopeptidase-N with Methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 260.664, 62.871, 81.620 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.020 |
| R-factor | 0.20716 |
| Rwork | 0.205 |
| R-free | 0.24571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fke |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 84218 | 7619 |
| <I/σ(I)> | 21.43 | 3.62 |
| Completeness [%] | 98.9 | 90 |
| Redundancy | 7.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 18% PEG3350, 200 MM LITHIUM SULFATE, 100 MM HEPES |
