6BRH
The SAM domain of mouse SAMHD1 is critical for its activation and regulation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 112.031, 130.053, 90.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.400 |
| R-factor | 0.19856 |
| Rwork | 0.196 |
| R-free | 0.24673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aja |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.141 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 18463 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.4 | 298 | 100 mM SPG (Qiagen) buffer, pH 7.4, 25% PEG 1500 |
