6BRH
The SAM domain of mouse SAMHD1 is critical for its activation and regulation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 112.031, 130.053, 90.765 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.400 |
R-factor | 0.19856 |
Rwork | 0.196 |
R-free | 0.24673 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5aja |
RMSD bond length | 0.010 |
RMSD bond angle | 1.141 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 18463 | |
<I/σ(I)> | 10.7 | |
Completeness [%] | 98.9 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.4 | 298 | 100 mM SPG (Qiagen) buffer, pH 7.4, 25% PEG 1500 |